Hotspot

psaJ-rpl33

LSC · 66222-66653 · 432 bp

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Pi 0.0605
MarkerSeek score 78.60
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
432
variable_sites
25
pi
0.0605
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TCCAGATGCATTAACATTTCCCT CGAACATCTTTACCCTTTGCC 39.1 47.6 58.1 57.5 477-486 bp 1.0 50.4
psaJ-rpl33_p2 TCCAGATGCATTAACATTTCCC CGAACATCTTTACCCTTTGCC 40.9 47.6 56.7 57.5 477-486 bp 1.0 49.2
psaJ-rpl33_p3 CCAGATGCATTAACATTTCCCT CGAACATCTTTACCCTTTGCC 40.9 47.6 56.7 57.5 476-485 bp 1.0 49.2
psaJ-rpl33_p4 TTCCAGATGCATTAACATTTCCC CGAACATCTTTACCCTTTGCC 39.1 47.6 57.4 57.5 478-487 bp 1.0 47.3
psaJ-rpl33_p5 CCAGATGCATTAACATTTCCCTT CGAACATCTTTACCCTTTGCC 39.1 47.6 57.4 57.5 476-485 bp 1.0 47.3