Hotspot

ycf1

SSC · 128352-133928 · 5577 bp

Back to Parrotia

Pi 0.00305

MarkerSeek score 40.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5577
variable_sites: 17
pi: 0.00305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAAGCTGGGTGGATACGTA	GAGGATCCGGACAAAATCGA	50.0	50.0	58.4	57.4	304-352 bp	1.0	72.7
ycf1_p2	GTTGACAAGCTGGGTGGATA	GAGGATCCGGACAAAATCGA	50.0	50.0	57.5	57.4	308-356 bp	1.0	72.3
ycf1_p3	TTGACAAGCTGGGTGGATAC	GAGGATCCGGACAAAATCGA	50.0	50.0	57.5	57.4	307-355 bp	1.0	72.3
ycf1_p4	AAAGTTGACAAGCTGGGTGG	GAGGATCCGGACAAAATCGA	50.0	50.0	58.6	57.4	311-359 bp	1.0	71.4
ycf1_p5	CAAGCTGGGTGGATACGTAA	GAGGATCCGGACAAAATCGA	50.0	50.0	57.3	57.4	303-351 bp	1.0	70.7