Hotspot

trnL-UAG-ccsA

SSC · 118748-118846 · 99 bp

Back to Parrotia

Pi 0.0206

MarkerSeek score 66.30

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 99
variable_sites: 2
pi: 0.0206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	ACAATTGAAACAATCGAAAAGGAGA	50.0	32.0	57.9	58.0	220-222 bp	1.0	41.4
trnL-UAG-ccsA_p2	AAATTGGTAGACACGCTGCT	ACAATTGAAACAATCGAAAAGGAGA	45.0	32.0	57.8	58.0	227-229 bp	1.0	41.4
trnL-UAG-ccsA_p3	GGTAGACACGCTGCTCTTAG	ACAATTGAAACAATCGAAAAGGAGA	55.0	32.0	57.8	58.0	222-224 bp	1.0	41.4
trnL-UAG-ccsA_p4	GTAGACACGCTGCTCTTAGG	ACAATTGAAACAATCGAAAAGGAGA	55.0	32.0	57.8	58.0	221-223 bp	1.0	41.4
trnL-UAG-ccsA_p5	AATTGGTAGACACGCTGCTC	ACAATTGAAACAATCGAAAAGGAGA	50.0	32.0	58.3	58.0	226-228 bp	1.0	41.4