Hotspot

ndhF-rpl32

SSC · 116355-117436 · 1082 bp

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Pi 0.00755
MarkerSeek score 54.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1082
variable_sites
8
pi
0.00755
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCGTCGGAAAAAGGAGAAGT TCCAAAAATTTTTACGAATACGCT 50.0 29.2 57.8 56.2 1215-1237 bp 1.0 42.0
ndhF-rpl32_p2 CTGGAAGTGGAACGAAAGGT TCCAAAAATTTTTACGAATACGCT 50.0 29.2 57.7 56.2 1173-1195 bp 1.0 42.0
ndhF-rpl32_p3 CCGTCGGAAAAAGGAGAAGT TCCAAAAATTTTTACGAATACGC 50.0 30.4 57.8 54.8 1215-1237 bp 1.0 42.0
ndhF-rpl32_p4 CCGTCGGAAAAAGGAGAAGT TCCAAAAATTTTTACGAATACGCTT 50.0 28.0 57.8 56.8 1215-1237 bp 1.0 42.0
ndhF-rpl32_p5 TTTGTTGCCGTCGGAAAAAG TCCAAAAATTTTTACGAATACGCT 45.0 29.2 57.8 56.2 1222-1244 bp 1.0 41.9