Hotspot

rps19-rpl2

IRb · 87839-87910 · 72 bp

Back to Parrotia

Pi 0.0317

MarkerSeek score 73.00

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 72
variable_sites: 2
pi: 0.0317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TCCGCCTTTGTGTTAAGCTT	GGGCCCCAATTGGTAGAAAA	45.0	50.0	58.0	58.1	262-271 bp	1.0	82.7
rps19-rpl2_p2	TTCCGCCTTTGTGTTAAGCT	GGGCCCCAATTGGTAGAAAA	45.0	50.0	58.0	58.1	263-272 bp	1.0	82.7
rps19-rpl2_p3	TCCGCCTTTGTGTTAAGCTT	AGAAAAAGACCCACAACCCC	45.0	50.0	58.0	57.9	248-257 bp	1.0	82.6
rps19-rpl2_p4	TCCGCCTTTGTGTTAAGCTT	GAAAAAGACCCACAACCCCT	45.0	50.0	58.0	57.9	247-256 bp	1.0	82.6
rps19-rpl2_p5	TTCCGCCTTTGTGTTAAGCT	GAAAAAGACCCACAACCCCT	45.0	50.0	58.0	57.9	248-257 bp	1.0	82.6