Hotspot

petA-psbJ

LSC · 66673-67725 · 1053 bp

Back to Parrotia

Pi 0.00381
MarkerSeek score 61.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1053
variable_sites
4
pi
0.00381
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTGGC ACTGGTATTCCTGTGATCGG 50.0 50.0 57.8 56.7 1230 bp 1.0 69.0
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC CTGGTATTCCTGTGATCGGT 50.0 50.0 57.8 56.7 1229 bp 1.0 69.0
petA-psbJ_p3 GATCCATTACGGGTCCAAGG ACTGGTATTCCTGTGATCGG 55.0 50.0 57.7 56.7 1248 bp 1.0 68.2
petA-psbJ_p4 GATCCATTACGGGTCCAAGG CTGGTATTCCTGTGATCGGT 55.0 50.0 57.7 56.7 1247 bp 1.0 68.2
petA-psbJ_p5 GGTCCAAGGCCTTTTGTTCT CTGGTATTCCTGTGATCGGT 50.0 50.0 58.3 56.7 1236 bp 1.0 67.9