Hotspot

trnF-GAA-ndhJ

LSC · 51253-51958 · 706 bp

Back to Parrotia

Pi 0.00863

MarkerSeek score 65.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 6
pi: 0.00863
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	838-848 bp	1.0	80.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	802-812 bp	1.0	80.6
trnF-GAA-ndhJ_p3	TGAGGATGATACGTCGGGAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.9	58.1	863-873 bp	1.0	80.4
trnF-GAA-ndhJ_p4	GGATGATACGTCGGGAATGG	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	860-870 bp	1.0	80.2
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	ACGCCTGAAACGCATCTTAA	55.0	45.0	58.0	58.2	866-876 bp	1.0	80.0