Hotspot

trnE-UUC-trnT-GGU

LSC · 33488-34364 · 877 bp

Back to Parrotia

Pi 0.00457

MarkerSeek score 61.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 877
variable_sites: 4
pi: 0.00457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1017-1018 bp	1.0	79.1
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.8	57.9	982-983 bp	1.0	78.3
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	979-980 bp	1.0	78.3
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	1014-1015 bp	1.0	77.9
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.3	57.9	944-945 bp	1.0	77.5