Hotspot

trnC-GCA-petN

LSC · 29414-30255 · 842 bp

Back to Parrotia

Pi 0.00475

MarkerSeek score 61.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 4
pi: 0.00475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACGAGCGAAAGGGAAAATGT	50.0	45.0	58.1	58.1	946 bp	1.0	78.8
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CCATACTACGAGCGAAAGGG	50.0	55.0	58.1	57.9	953 bp	1.0	78.6
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CCCATACTACGAGCGAAAGG	50.0	55.0	58.1	57.9	954 bp	1.0	78.6
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CCCCATACTACGAGCGAAAG	50.0	55.0	58.1	57.9	955 bp	1.0	78.6
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	908 bp	1.0	78.5