Hotspot

ycf1

SSC · 120909-126551 · 5643 bp

Back to Paronychia

Pi 0.0552

MarkerSeek score 67.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5643
variable_sites: 310
pi: 0.0552
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGCGCGTCATCTTCTTCATA	GGAATCGTCCAACGCGATAT	50.0	50.0	58.1	58.2	823-829 bp	1.0	82.6
ycf1_p2	AACCCGCATGGAACCTTTTA	GGAATCGTCCAACGCGATAT	45.0	50.0	57.7	58.2	992 bp	0.5	55.9
ycf1_p3	GGCGCGTCATCTTCTTCATA	TTTCACCCAACGAAACTGCA	50.0	45.0	58.1	58.5	403 bp	0.5	54.5
ycf1_p4	GGCGCGTCATCTTCTTCATA	TTTTCACCCAACGAAACTGC	50.0	45.0	58.1	57.4	404 bp	0.5	54.3
ycf1_p5	ACCCGCATGGAACCTTTTAT	GGAATCGTCCAACGCGATAT	45.0	50.0	57.5	58.2	991 bp	0.5	54.0