Hotspot

ndhF

SSC · 107650-109887 · 2238 bp

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Pi 0.0493

MarkerSeek score 71.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 110
pi: 0.0493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGCTGGGGAATCAACATTGA	ACGAATCCGCTTTTTGGAAA	45.0	40.0	57.4	56.5	2958 bp	0.5	43.5
ndhF_p2	TGAGTCAATTTGCTGGGGAA	ACGAATCCGCTTTTTGGAAA	45.0	40.0	57.3	56.5	2968 bp	0.5	43.0
ndhF_p3	TGCTGGGGAATCAACATTGA	TTTTAACGCCGCCCAATAGC	45.0	50.0	57.4	59.5	3000 bp	0.5	42.8
ndhF_p4	GCTGGGGAATCAACATTGAA	CTTTTAACGCCGCCCAATAG	45.0	50.0	56.3	57.5	3000 bp	0.5	42.5
ndhF_p5	ATGAGTCAATTTGCTGGGGA	ACGAATCCGCTTTTTGGAAA	45.0	40.0	57.1	56.5	2969 bp	0.5	41.1