Hotspot

trnI-CAU-rpl23

IRa · 149146-149310 · 165 bp

Back to Paronychia

Pi 0.123
MarkerSeek score 79.60
Primer available yes
Label trnI-CAU-rpl23

Feature Metadata

label_name
trnI-CAU-rpl23
feature_type
igs
region
IRa
length_bp
165
variable_sites
19
pi
0.123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-rpl23_p1 GAATGGTTAAAGCGCCCAAC ACTGCATATTTGATTCCATCCA 50.0 36.4 58.0 55.8 250 bp 0.5 26.5
trnI-CAU-rpl23_p2 GCTAAGCATCCATGGCTGAA ACTGCATATTTGATTCCATCCA 50.0 36.4 58.3 55.8 267 bp 0.5 24.0
trnI-CAU-rpl23_p3 AATGGTTAAAGCGCCCAACT ACTGCATATTTGATTCCATCCA 45.0 36.4 58.4 55.8 249 bp 0.5 23.6
trnI-CAU-rpl23_p4 TGAATGGTTAAAGCGCCCAA ACTGCATATTTGATTCCATCCA 45.0 36.4 58.4 55.8 251 bp 0.5 23.5
trnI-CAU-rpl23_p5 TGGCGAATTCGTAGGTTCAA ACTGCATATTTGATTCCATCCA 45.0 36.4 57.5 55.8 223 bp 0.5 22.9