Hotspot

rpl33-rps18

LSC · 65502-65794 · 293 bp

Back to Paronychia

Pi 0.126
MarkerSeek score 77.50
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
293
variable_sites
36
pi
0.126
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 ACAAACATACAATTCATGGAGAGA AGGGGTCGCTTGGATTTATC 33.3 50.0 56.4 57.4 351-403 bp 1.0 49.3
rpl33-rps18_p2 ACAAACATACAATTCATGGAGAG AGGGGTCGCTTGGATTTATC 34.8 50.0 55.0 57.4 351-403 bp 1.0 49.3
rpl33-rps18_p3 ACAAACATACAATTCATGGAGAGA AAGGGGTCGCTTGGATTTATC 33.3 47.6 56.4 58.1 352-404 bp 1.0 49.3
rpl33-rps18_p4 ACAAACATACAATTCATGGAGAGA AAGGGGTCGCTTGGATTTAT 33.3 45.0 56.4 56.9 352-404 bp 1.0 49.3
rpl33-rps18_p5 ACAAACATACAATTCATGGAGAGA ATAAGGGGTCGCTTGGATTT 33.3 45.0 56.4 56.9 354-406 bp 1.0 49.3