Hotspot

ndhC-trnV-UAC

LSC · 48230-49105 · 876 bp

Back to Paronychia

Pi 0.0482

MarkerSeek score 79.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 876
variable_sites: 40
pi: 0.0482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGCCCAGAAAATATCATATTCA	CCGAGAAAGTCTACGGTTCG	31.8	55.0	53.3	58.1	948-1100 bp	1.0	46.7
ndhC-trnV-UAC_p2	TCCGGAAAATAGAAATGCTAA	GTTCGAGTCCGTATAGCCCT	33.3	55.0	52.9	58.7	984-1136 bp	1.0	46.5
ndhC-trnV-UAC_p3	TCCGGAAAATAGAAATGCTAA	CCGAGAAAGTCTACGGTTCG	33.3	55.0	52.9	58.1	999-1151 bp	1.0	46.4
ndhC-trnV-UAC_p4	TCCGGAAAATAGAAATGCTAAA	CCGAGAAAGTCTACGGTTCG	31.8	55.0	53.8	58.1	999-1151 bp	1.0	46.4
ndhC-trnV-UAC_p5	TCCGGAAAATAGAAATGCTAAAA	CCGAGAAAGTCTACGGTTCG	30.4	55.0	54.6	58.1	999-1151 bp	1.0	46.4