Hotspot

trnF-GAA-ndhJ

LSC · 46146-46553 · 408 bp

Back to Paronychia

Pi 0.0825
MarkerSeek score 81.80
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
408
variable_sites
33
pi
0.0825
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA CCCGAAAGTTGGATAGGGTG 55.0 55.0 58.0 58.0 556-814 bp 1.0 84.5
trnF-GAA-ndhJ_p2 GGGATAGCTCAGCTGGTAGA CCGAAAGTTGGATAGGGTGG 55.0 55.0 58.0 58.0 555-813 bp 1.0 84.5
trnF-GAA-ndhJ_p3 CAGTAAAGGGGAATGGTCGG CCCGAAAGTTGGATAGGGTG 55.0 55.0 58.0 58.0 574-832 bp 1.0 84.1
trnF-GAA-ndhJ_p4 CAGTAAAGGGGAATGGTCGG CCGAAAGTTGGATAGGGTGG 55.0 55.0 58.0 58.0 573-831 bp 1.0 84.1
trnF-GAA-ndhJ_p5 CCAGTAAAGGGGAATGGTCG CCGAAAGTTGGATAGGGTGG 55.0 55.0 58.0 58.0 574-832 bp 1.0 84.1