Hotspot

trnT-UGU-trnL-UAA

LSC · 44380-45042 · 663 bp

Back to Paronychia

Pi 0.0736
MarkerSeek score 81.80
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
663
variable_sites
47
pi
0.0736
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG AGCCAATTAAGTCCGTAGCG 55.0 50.0 57.9 58.1 736-741 bp 1.0 86.1
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 724-729 bp 1.0 84.8
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG GCCAATTAAGTCCGTAGCGT 55.0 50.0 57.9 58.4 735-740 bp 1.0 83.8
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 718-723 bp 1.0 83.5
trnT-UGU-trnL-UAA_p5 CTAACCTCTGAGCTAAGCGG AGCCAATTAAGTCCGTAGCG 55.0 50.0 57.5 58.1 728-733 bp 1.0 83.3