Hotspot

trnG-GCC-trnfM-CAU

LSC · 34588-34791 · 204 bp

Back to Paronychia

Pi 0.108

MarkerSeek score 83.00

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 204
variable_sites: 21
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	282-286 bp	1.0	87.1
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.8	58.1	276-280 bp	1.0	86.7
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TTTGTCGTCGATTCACTCCC	50.0	50.0	57.8	57.9	361-363 bp	1.0	86.2
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TCACGGGTTCAAATCCTGTC	50.0	50.0	57.8	57.8	275-279 bp	1.0	86.2
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TCGTCGATTCACTCCCAGTA	50.0	50.0	57.8	57.9	357-359 bp	1.0	86.0