Hotspot

psbM-trnD-GUC

LSC · 28311-28958 · 648 bp

Back to Paronychia

Pi 0.0844

MarkerSeek score 86.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 648
variable_sites: 51
pi: 0.0844
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAAGAGTGCAGTAGC	TCAATTGGTTAGAGCACCGC	45.0	50.0	55.2	58.6	743-758 bp	1.0	62.5
psbM-trnD-GUC_p2	ACTAGAATGAAGAGTGCAGTAGC	TCAATTGGTTAGAGCACCGC	43.5	50.0	58.0	58.6	746-761 bp	1.0	61.0
psbM-trnD-GUC_p3	AGGAACTAGAATGAAGAGTGCAG	TCAATTGGTTAGAGCACCGC	43.5	50.0	57.9	58.6	750-765 bp	1.0	60.3
psbM-trnD-GUC_p4	AGGAACTAGAATGAAGAGTGCA	TCAATTGGTTAGAGCACCGC	40.9	50.0	56.8	58.6	750-765 bp	1.0	59.6
psbM-trnD-GUC_p5	GGAACTAGAATGAAGAGTGCAGT	TCAATTGGTTAGAGCACCGC	43.5	50.0	58.2	58.6	749-764 bp	1.0	59.6