Hotspot

trnS-GCU-trnG-UCC

LSC · 7668-8236 · 569 bp

Back to Paronychia

Pi 0.0935

MarkerSeek score 82.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 569
variable_sites: 50
pi: 0.0935
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TCCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCACT	57.9	38.1	58.1	55.5	622-949 bp	1.0	51.5
trnS-GCU-trnG-UCC_p2	TCCACTCAGCCATCTCTCC	AGAACGAATCACACTTTTACCAC	57.9	39.1	58.1	57.3	625-952 bp	1.0	50.4
trnS-GCU-trnG-UCC_p3	TCCACTCAGCCATCTCTCC	GAACGAATCACACTTTTACCACT	57.9	39.1	58.1	57.3	624-951 bp	1.0	50.4
trnS-GCU-trnG-UCC_p4	TCCACTCAGCCATCTCTCC	AGGGGTTAATAGAACGAATCACA	57.9	39.1	58.1	57.3	635-962 bp	1.0	50.2
trnS-GCU-trnG-UCC_p5	TCCACTCAGCCATCTCTCC	GGGGTTAATAGAACGAATCACAC	57.9	43.5	58.1	57.3	634-961 bp	1.0	49.8