Hotspot

ndhF

SSC · 107806-110049 · 2244 bp

Back to Parnassia

Pi 0.0167

MarkerSeek score 59.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 202
pi: 0.0167
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TAACTGTCCTAGCCCTACCG	AGGGACAAGAACGAGTGAGT	55.0	50.0	57.9	58.3	2438-2977 bp	0.884615	84.5
ndhF_p2	GCTCGGGTTATGGAAGAAGG	AGGGACAAGAACGAGTGAGT	55.0	50.0	58.0	58.3	2568-2946 bp	0.846154	82.6
ndhF_p3	CACATTTTCCATCGGACCCT	AGGGACAAGAACGAGTGAGT	50.0	50.0	57.9	58.3	2608-2986 bp	0.846154	81.8
ndhF_p4	ACCACATTTTCCATCGGACC	AGGGACAAGAACGAGTGAGT	50.0	50.0	58.2	58.3	2610-2988 bp	0.846154	81.5
ndhF_p5	GGTATCAGCAACAACAGGGT	AGGGACAAGAACGAGTGAGT	50.0	50.0	57.8	58.3	2536-2914 bp	0.846154	81.4