Hotspot

ccsA-ndhD

SSC · 112848-113074 · 227 bp

Back to Parnassia

Pi 0.0519

MarkerSeek score 74.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 227
variable_sites: 57
pi: 0.0519
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGAATAGGACTGCATAGTTATGGT	CGTTGACAAGGTTGAGGCTA	41.7	50.0	57.9	57.8	292-320 bp	1.0	57.8
ccsA-ndhD_p2	GGAATAGGACTGCATAGTTATGGT	ATCCGTTGACAAGGTTGAGG	41.7	50.0	57.9	57.8	295-323 bp	1.0	57.6
ccsA-ndhD_p3	AGGACTGCATAGTTATGGTT	CGTTGACAAGGTTGAGGCTA	40.0	50.0	53.7	57.8	287-315 bp	1.0	56.3
ccsA-ndhD_p4	AGGACTGCATAGTTATGGTT	ATCCGTTGACAAGGTTGAGG	40.0	50.0	53.7	57.8	290-318 bp	1.0	56.2
ccsA-ndhD_p5	AGGAATAGGACTGCATAGTTATGG	CGTTGACAAGGTTGAGGCTA	41.7	50.0	57.6	57.8	293-321 bp	1.0	55.8