Hotspot

psbE-petL

LSC · 63587-64881 · 1295 bp

Back to Parnassia

Pi 0.0245

MarkerSeek score 74.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1295
variable_sites: 188
pi: 0.0245
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AACGCCGCTAGTAGAAAAGC	50.0	50.0	57.8	58.6	1005-1389 bp	1.0	85.6
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AACGCCGCTAGTAGAAAAGC	55.0	50.0	58.2	58.6	1000-1384 bp	1.0	85.3
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	AACGCCGCTAGTAGAAAAGC	50.0	50.0	58.4	58.6	998-1382 bp	1.0	84.1
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	ACGCCGCTAGTAGAAAAGC	50.0	52.6	57.8	58.0	1004-1388 bp	1.0	82.4
psbE-petL_p5	TTCTCCTGTGCTTCCAGACA	AACGCCGCTAGTAGAAAAGC	50.0	50.0	58.6	58.6	999-1383 bp	1.0	82.2