Hotspot

atpB-rbcL

LSC · 54442-55313 · 872 bp

Back to Parnassia

Pi 0.029

MarkerSeek score 80.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 872
variable_sites: 129
pi: 0.029
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATTTGAGCGATACGTCCGAG	CCAGCCTTGAATCCAACACT	50.0	50.0	57.9	58.1	899-1014 bp	1.0	90.4
atpB-rbcL_p2	CCAGGCCAGAAGTAGTAGGA	CCAGCCTTGAATCCAACACT	55.0	50.0	57.8	58.1	853-968 bp	1.0	90.0
atpB-rbcL_p3	TCCAGGCCAGAAGTAGTAGG	CCAGCCTTGAATCCAACACT	55.0	50.0	57.8	58.1	854-969 bp	1.0	90.0
atpB-rbcL_p4	ATTTGAGCGATACGTCCGAG	AGCCTTGAATCCAACACTCG	50.0	50.0	57.9	58.2	897-1012 bp	1.0	89.5
atpB-rbcL_p5	ATTTGAGCGATACGTCCGAG	CCTTGAATCCAACACTCGCT	50.0	50.0	57.9	58.2	895-1010 bp	1.0	89.5