Hotspot

psbZ-trnG-GCC

LSC · 35852-36677 · 826 bp

Back to Parnassia

Pi 0.0296

MarkerSeek score 74.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 143
pi: 0.0296
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CAAGACCGGAGCTATCAACC	CCGGAAGACCAAGAGCTAAC	55.0	55.0	58.1	58.0	1830-2207 bp	1.0	91.4
psbZ-trnG-GCC_p2	CAAGACCGGAGCTATCAACC	CGTTGTTTTTCGACCGGAAG	55.0	50.0	58.1	58.0	1843-2220 bp	1.0	91.4
psbZ-trnG-GCC_p3	ACCCCATGTCCGAATAAAGC	CCGGAAGACCAAGAGCTAAC	50.0	55.0	57.9	58.0	1565-1934 bp	0.923077	88.4
psbZ-trnG-GCC_p4	ACCCCATGTCCGAATAAAGC	CGTTGTTTTTCGACCGGAAG	50.0	50.0	57.9	58.0	1578-1947 bp	0.923077	88.4
psbZ-trnG-GCC_p5	ACCCCATGTCCGAATAAAGC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.9	58.0	1285-1654 bp	0.923077	88.3