Hotspot

trnT-GGU-psbD

LSC · 31200-32493 · 1294 bp

Back to Parnassia

Pi 0.0275

MarkerSeek score 73.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1294
variable_sites: 185
pi: 0.0275
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	TTTACCAAGGGCTATAGTCA	55.0	40.0	58.1	53.0	1227-1500 bp	1.0	52.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.1	53.0	1230-1503 bp	1.0	52.0
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.3	53.0	1204-1477 bp	1.0	52.0
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	TTACCAAGGGCTATAGTCAT	55.0	40.0	58.1	52.8	1226-1499 bp	1.0	52.0
trnT-GGU-psbD_p5	GGCCCTTTTAACTCAGTGGT	TTTACCAAGGGCTATAGTCA	50.0	40.0	57.7	53.0	1328-1515 bp	0.961538	50.5