Hotspot

psbM-trnD-GUC

LSC · 28816-29669 · 854 bp

Back to Parnassia

Pi 0.0265
MarkerSeek score 80.30
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
854
variable_sites
111
pi
0.0265
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 CCTTGACTTTGGCTGATGGT CCGTCTACTTGATTTCCCCG 50.0 55.0 58.1 58.1 729-1099 bp 1.0 90.8
psbM-trnD-GUC_p2 CCTTGACTTTGGCTGATGGT CGTCTACTTGATTTCCCCGG 50.0 55.0 58.1 58.1 728-1098 bp 1.0 90.8
psbM-trnD-GUC_p3 CCTTGACTTTGGCTGATGGT ACTTGATTTCCCCGGGATTG 50.0 50.0 58.1 57.9 723-1093 bp 1.0 90.2
psbM-trnD-GUC_p4 CCTTGACTTTGGCTGATGGT CTTGATTTCCCCGGGATTGT 50.0 50.0 58.1 57.9 722-1092 bp 1.0 90.2
psbM-trnD-GUC_p5 TATCGCCTTGACTTTGGCTG CGTCTACTTGATTTCCCCGG 50.0 55.0 58.3 58.1 733-1073 bp 0.923077 86.3