Hotspot

atpH-atpI

LSC · 13335-13966 · 632 bp

Back to Parnassia

Pi 0.031
MarkerSeek score 74.80
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
632
variable_sites
107
pi
0.031
unique_haplotype_count
25
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 CTCGCGATACCTTCTACAGC GAGTTACCTCAGGGGGAGTT 55.0 55.0 58.0 58.0 1038-1195 bp 1.0 91.3
atpH-atpI_p2 CTCGCGATACCTTCTACAGC GCGAATCCATGGAAGGTCAT 55.0 50.0 58.0 58.0 680-837 bp 0.961538 89.9
atpH-atpI_p3 CTCGCGATACCTTCTACAGC TCCACAAACCATTCCCACTG 55.0 50.0 58.0 58.0 1219-1376 bp 0.923077 88.5
atpH-atpI_p4 GCAGCAATAACAGAAGCAGC TCCACAAACCATTCCCACTG 50.0 50.0 58.1 58.0 1135-1292 bp 0.923077 88.1
atpH-atpI_p5 ACCCGCGGAAAGAAAAGAAT TCCACAAACCATTCCCACTG 45.0 50.0 58.1 58.0 1677-1849 bp 0.923077 87.4