Hotspot

ndhF

SSC · 115244-117478 · 2235 bp

Back to Parkinsonia

Pi 0.0094

MarkerSeek score 48.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 21
pi: 0.0094
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGTTTCGATGCAACAACCCA	TTGACCCATTTGCACTTCGT	45.0	45.0	58.6	58.3	2748 bp	0.5	52.6
ndhF_p2	ATGTTTCGATGCAACAACCC	TTGACCCATTTGCACTTCGT	45.0	45.0	57.3	58.3	2749 bp	0.5	51.7
ndhF_p3	GTTTCGATGCAACAACCCAT	TTGACCCATTTGCACTTCGT	45.0	45.0	57.3	58.3	2747 bp	0.5	51.7
ndhF_p4	TGTTTCGATGCAACAACCCA	TGACCCATTTGCACTTCGTA	45.0	45.0	58.6	57.4	2747 bp	0.5	50.7
ndhF_p5	TGTTTCGATGCAACAACCCA	ACCCATTTGCACTTCGTACT	45.0	45.0	58.6	57.4	2745 bp	0.5	50.7