Hotspot

trnN-GUU-trnR-ACG

IRa · 134858-135470 · 613 bp

Back to Parkinsonia

Pi 0.0261

MarkerSeek score 73.80

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 613
variable_sites: 16
pi: 0.0261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	754-755 bp	1.0	80.0
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	791-792 bp	1.0	79.3
trnN-GUU-trnR-ACG_p3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	795-796 bp	1.0	79.1
trnN-GUU-trnR-ACG_p4	CCGTTGTTCCTCAGTAGCTC	TTTTAGGGTACTCCGGGGAA	55.0	50.0	58.0	57.7	808-809 bp	1.0	78.8
trnN-GUU-trnR-ACG_p5	CCGTTGTTCCTCAGTAGCTC	TTTTTAGGGTACTCCGGGGA	55.0	50.0	58.0	57.7	809-810 bp	1.0	78.8