Hotspot

trnR-ACG-trnN-GUU

IRb · 113451-114063 · 613 bp

Back to Parkinsonia

Pi 0.0261

MarkerSeek score 73.80

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 613
variable_sites: 16
pi: 0.0261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	754-755 bp	1.0	80.0
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	791-792 bp	1.0	79.3
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	795-796 bp	1.0	79.1
trnR-ACG-trnN-GUU_p4	TTTTAGGGTACTCCGGGGAA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	57.7	58.0	808-809 bp	1.0	78.8
trnR-ACG-trnN-GUU_p5	TTTTTAGGGTACTCCGGGGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	57.7	58.0	809-810 bp	1.0	78.8