Hotspot

accD-psaI

LSC · 63325-63796 · 472 bp

Back to Parkinsonia

Pi 0.067
MarkerSeek score 75.10
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
472
variable_sites
31
pi
0.067
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GCTCCATGCTTTCTTTCCTT AAGTTGCTGAGAGTTGTCAT 45.0 40.0 56.3 54.9 526-710 bp 1.0 46.7
accD-psaI_p2 GCTCCATGCTTTCTTTCCTTT AAGTTGCTGAGAGTTGTCAT 42.9 40.0 57.1 54.9 526-710 bp 1.0 45.2
accD-psaI_p3 GCTCCATGCTTTCTTTCCTTTG AAGTTGCTGAGAGTTGTCAT 45.5 40.0 58.4 54.9 526-710 bp 1.0 45.2
accD-psaI_p4 GTGAGTTATTTCAGCTCCATGC AAGTTGCTGAGAGTTGTCAT 45.5 40.0 57.7 54.9 539-723 bp 1.0 45.1
accD-psaI_p5 TGAGTTATTTCAGCTCCATGC AAGTTGCTGAGAGTTGTCAT 42.9 40.0 56.3 54.9 538-722 bp 1.0 45.1