Hotspot

psbZ-trnG-GCC

LSC · 39823-40218 · 396 bp

Back to Parkinsonia

Pi 0.0661

MarkerSeek score 83.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 396
variable_sites: 25
pi: 0.0661
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.0	57.7	551-596 bp	1.0	83.3
psbZ-trnG-GCC_p2	CTTCTCCTGATGGTTGGTCG	AAAAAGGAGAGGGGCGGATA	55.0	50.0	58.0	58.4	586-631 bp	1.0	82.3
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	554-599 bp	1.0	81.7
psbZ-trnG-GCC_p4	TCCTGATGGTTGGTCGAGTA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	547-592 bp	1.0	81.7
psbZ-trnG-GCC_p5	GCTTCTCCTGATGGTTGGTC	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.3	57.7	552-597 bp	1.0	81.4