Hotspot

trnC-GCA-petN

LSC · 30027-31102 · 1076 bp

Back to Parkinsonia

Pi 0.0359

MarkerSeek score 75.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1076
variable_sites: 37
pi: 0.0359
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1132-1261 bp	1.0	83.7
trnC-GCA-petN_p2	GTATCATTTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	1184-1313 bp	1.0	83.0
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1193-1322 bp	1.0	81.8
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCTTCCCCATACTACGAGGG	50.0	55.0	58.1	57.6	1183-1312 bp	1.0	81.7
trnC-GCA-petN_p5	GTATCATTTTGGCGGCATGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.9	57.6	1245-1374 bp	1.0	81.2