Hotspot

trnK-UUU-rps16

LSC · 4416-5694 · 1279 bp

Back to Parkinsonia

Pi 0.0526

MarkerSeek score 80.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1279
variable_sites: 63
pi: 0.0526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGATGGTATGGACGAATCC	GGTGTTTTTATGGAACTTTGTCCT	55.0	37.5	57.9	57.8	1401-1421 bp	1.0	53.7
trnK-UUU-rps16_p2	CCGATGGTATGGACGAATCC	TGGAACTTTGTCCTAATCAACA	55.0	36.4	57.9	55.6	1391-1411 bp	1.0	51.5
trnK-UUU-rps16_p3	CCGATGGTATGGACGAATCC	ACTTTGTCCTAATCAACAAACCA	55.0	34.8	57.9	56.6	1387-1407 bp	1.0	51.4
trnK-UUU-rps16_p4	CCGATGGTATGGACGAATCC	GGTGTTTTTATGGAACTTTGTCC	55.0	39.1	57.9	56.5	1401-1421 bp	1.0	51.0
trnK-UUU-rps16_p5	AGTCGCACTTAAAAGCCGAG	GGTGTTTTTATGGAACTTTGTCCT	50.0	37.5	58.6	57.8	1352-1372 bp	1.0	49.9