Hotspot

rps19

LSC · 90916-91194 · 279 bp

Back to Parkia

Pi 0
MarkerSeek score 33.90
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
LSC
length_bp
279
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.62
markerseek_score
33.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 TTGTCCCATAATCCCCCACT GATGAAGGAACGGGAAGACC 50.0 55.0 58.0 58.0 1433 bp 0.5 59.8
rps19_p2 TTGTCCCATAATCCCCCACT CCAGATGAAGGAACGGGAAG 50.0 55.0 58.0 58.0 1436 bp 0.5 59.7
rps19_p3 TTGTCCCATAATCCCCCACT GGGCCCCAATTGGTAGAAAA 50.0 50.0 58.0 58.1 895 bp 0.5 59.5
rps19_p4 TTATCCGTGCAATTCCCTCG GATGAAGGAACGGGAAGACC 50.0 55.0 58.1 58.0 1614 bp 0.5 59.4
rps19_p5 TTATCCGTGCAATTCCCTCG CCAGATGAAGGAACGGGAAG 50.0 55.0 58.1 58.0 1617 bp 0.5 59.3