Hotspot

ndhA

SSC · 126428-128956 · 2529 bp

Pi 0.00718

MarkerSeek score 47.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2529
variable_sites: 18
pi: 0.00718
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GATTTGCGTGGGCTAAGGTA	GTGAGAGTTGAAGCCCCAAA	50.0	50.0	58.0	58.0	2911-2913 bp	1.0	80.7
ndhA_p2	TTGCGTGGGCTAAGGTAATC	GTGAGAGTTGAAGCCCCAAA	50.0	50.0	58.0	58.0	2908-2910 bp	1.0	80.7
ndhA_p3	GATTTGCGTGGGCTAAGGTA	AAAAAGCCGTCTCCGACTTT	50.0	45.0	58.0	58.0	2956-2958 bp	1.0	80.6
ndhA_p4	TTGCGTGGGCTAAGGTAATC	AAAAAGCCGTCTCCGACTTT	50.0	45.0	58.0	58.0	2953-2955 bp	1.0	80.6
ndhA_p5	TGCAGCTCGTACTGTTTGTT	GTGAGAGTTGAAGCCCCAAA	45.0	50.0	58.1	58.0	2865-2867 bp	1.0	80.6