Hotspot

rbcL-accD

LSC · 62513-63540 · 1028 bp

Back to Parkia

Pi 0.0218
MarkerSeek score 71.90
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
1028
variable_sites
21
pi
0.0218
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AGTCCTGAATTAGCTGCTGC ACTTAGCCCACACCTGTATTC 50.0 47.6 58.0 57.4 1116-1169 bp 1.0 72.2
rbcL-accD_p2 AGTCCTGAATTAGCTGCTGC AGCCCACACCTGTATTCTAAC 50.0 47.6 58.0 57.4 1112-1165 bp 1.0 72.2
rbcL-accD_p3 AGTCCTGAATTAGCTGCTGC GCCCACACCTGTATTCTAACT 50.0 47.6 58.0 57.4 1111-1164 bp 1.0 72.2
rbcL-accD_p4 GAGGCTAGCAAATGGAGTCC AGCCCACACCTGTATTCTAAC 55.0 47.6 58.0 57.4 1127-1180 bp 1.0 72.1
rbcL-accD_p5 GAGGCTAGCAAATGGAGTCC GCCCACACCTGTATTCTAACT 55.0 47.6 58.0 57.4 1126-1179 bp 1.0 72.1