Hotspot

rpoB-trnC-GCA

LSC · 29605-30809 · 1205 bp

Back to Parkia

Pi 0.00338
MarkerSeek score 62.50
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1205
variable_sites
4
pi
0.00338
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 ACATTCCCGCATTTACACCC CTATTTTGTCGACCAGGCGA 50.0 50.0 58.5 58.0 1307-1318 bp 1.0 77.3
rpoB-trnC-GCA_p2 ACATTCCCGCATTTACACCC CCGGATTTGAACTGGGGAAA 50.0 50.0 58.5 58.1 1284-1295 bp 1.0 76.7
rpoB-trnC-GCA_p3 ACATTCCCGCATTTACACCC AGCTATTTTGTCGACCAGGC 50.0 50.0 58.5 58.3 1309-1320 bp 1.0 75.3
rpoB-trnC-GCA_p4 GCATTTACACCCCCAAGCAT CTATTTTGTCGACCAGGCGA 50.0 50.0 58.8 58.0 1299-1310 bp 1.0 75.1
rpoB-trnC-GCA_p5 GACATTCCCGCATTTACACC CTATTTTGTCGACCAGGCGA 50.0 50.0 57.2 58.0 1308-1319 bp 1.0 74.8