Hotspot

atpF-atpH

LSC · 14913-15546 · 634 bp

Back to Parkia

Pi 0.00476
MarkerSeek score 62.10
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
634
variable_sites
3
pi
0.00476
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 ATTAAACCCGAAACTCCCGG TGGGCTGGTTGTAGCATTAG 50.0 50.0 57.9 57.6 752-802 bp 1.0 76.4
atpF-atpH_p2 ATTAAACCCGAAACTCCCGG AGGCAGAGGGAAAAATACGG 50.0 50.0 57.9 57.6 819-869 bp 1.0 76.4
atpF-atpH_p3 ATTAAACCCGAAACTCCCGG GCAGAGGGAAAAATACGGGG 50.0 55.0 57.9 58.6 817-867 bp 1.0 74.8
atpF-atpH_p4 ATTAAACCCGAAACTCCCGG GGCAGAGGGAAAAATACGGG 50.0 55.0 57.9 58.6 818-868 bp 1.0 74.8
atpF-atpH_p5 ATTAAACCCGAAACTCCCGG CCGAGGCAGAGGGAAAAATA 50.0 50.0 57.9 57.3 822-872 bp 1.0 74.0