Hotspot

trnS-GCU-trnG-UCC

LSC · 9362-10319 · 958 bp

Back to Parkia

Pi 0.0171
MarkerSeek score 66.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
958
variable_sites
15
pi
0.0171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 1060-1070 bp 1.0 79.4
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 1059-1069 bp 1.0 79.4
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA TGAATCAAACGAGGGATCCC 50.0 50.0 58.2 57.3 1114-1124 bp 1.0 78.2
trnS-GCU-trnG-UCC_p4 TGGAAACGGAAAGAGAGGGA TGAATCAAACGAGGGATCCC 50.0 50.0 58.3 57.3 1119-1129 bp 1.0 77.2
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 1028-1038 bp 1.0 76.9