Pi
0.0211
MarkerSeek score
66.10
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | TAGCATAGCAAAGACGCGTT | CAAATGGAGTTGGGGTTGGA | 45.0 | 50.0 | 58.0 | 58.0 | 1319-2600 bp | 0.769231 | 82.7 |
| ycf1_p2 | CCCGGAACTTCTTCGTAGTG | CAAATGGAGTTGGGGTTGGA | 55.0 | 50.0 | 58.0 | 58.0 | 853-2174 bp | 0.769231 | 82.6 |
| ycf1_p3 | CCGGAACTTCTTCGTAGTGG | CAAATGGAGTTGGGGTTGGA | 55.0 | 50.0 | 58.0 | 58.0 | 852-2173 bp | 0.769231 | 82.6 |
| ycf1_p4 | GAGCGAGCCCTTTAATTCCA | CAAATGGAGTTGGGGTTGGA | 50.0 | 50.0 | 58.0 | 58.0 | 1055-2336 bp | 0.769231 | 82.4 |
| ycf1_p5 | GAGCTACTGAGGAACAACGG | CAAATGGAGTTGGGGTTGGA | 55.0 | 50.0 | 58.0 | 58.0 | 939-2220 bp | 0.74359 | 81.7 |
| ycf1_p1 | ATTTTGCGTCTTTGAGCACG | GAAAGCGGAAGAGGAAGAGG | 45.0 | 55.0 | 58.0 | 58.0 | 291-432 bp | 0.923077 | 88.5 |
| ycf1_p2 | CTGAATCCCGTCTGCTAACC | GAAAGCGGAAGAGGAAGAGG | 55.0 | 55.0 | 58.1 | 58.0 | 859-1135 bp | 0.923077 | 88.3 |
| ycf1_p3 | CAAAGCCAGTTCTTCCTCGT | GAAAGCGGAAGAGGAAGAGG | 50.0 | 55.0 | 58.1 | 58.0 | 217-358 bp | 0.923077 | 87.9 |
| ycf1_p4 | ATTTTGCGTCTTTGAGCACG | CCCAAGAAAAGCCAAACGTG | 45.0 | 50.0 | 58.0 | 58.1 | 204-345 bp | 0.923077 | 87.4 |
| ycf1_p5 | TCAAAGCCAGTTCTTCCTCG | GAAAGCGGAAGAGGAAGAGG | 50.0 | 55.0 | 57.8 | 58.0 | 218-359 bp | 0.923077 | 87.4 |