Hotspot

ycf1-trnN_GUU

IRa · 130912-131245 · 334 bp

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Pi 0.0515
MarkerSeek score 67.60
Primer available yes
Label ycf1-trnN_GUU

Feature Metadata

label_name
ycf1-trnN_GUU
feature_type
igs
region
IRa
length_bp
334
variable_sites
100
pi
0.0515
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN_GUU_p1 GGAAGAGATAAGAGGGCCCT TAGCATAGCAAAGACGCGTT 55.0 45.0 57.9 58.0 823-1649 bp 1.0 91.1
ycf1-trnN_GUU_p2 CCTTCTTCCATAACCCGAGC TAGCATAGCAAAGACGCGTT 55.0 45.0 58.0 58.0 851-1677 bp 0.974359 90.6
ycf1-trnN_GUU_p3 CCTTCTTCCATAACCCGAGC GGCAGAATAGCAGAGCAAGT 55.0 50.0 58.0 58.0 882-1708 bp 0.974359 90.3
ycf1-trnN_GUU_p4 CCTTCTTCCATAACCCGAGC GAGCGAGCCCTTTAATTCCA 55.0 50.0 58.0 58.0 587-1413 bp 0.974359 90.3
ycf1-trnN_GUU_p5 CCTTCTTCCATAACCCGAGC GTAACCCAGGCCAGAATCAG 55.0 55.0 58.0 58.0 999-1520 bp 0.923077 86.4