Hotspot

rpl32-trnL_UAG

SSC · 114298-115132 · 835 bp

Back to Paris

Pi 0.0166
MarkerSeek score 64.90
Primer available yes
Label rpl32-trnL_UAG

Feature Metadata

label_name
rpl32-trnL_UAG
feature_type
igs
region
SSC
length_bp
835
variable_sites
82
pi
0.0166
unique_haplotype_count
32
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL_UAG_p1 ACTCCTTTTTGAATGGCGGT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 1019-1053 bp 0.974359 88.6
rpl32-trnL_UAG_p2 ACTCCTTTTTGAATGGCGGT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 1020-1054 bp 0.974359 88.5
rpl32-trnL_UAG_p3 ACTCCTTTTTGAATGGCGGT TGGCGAAACCTTGGGTAAAA 45.0 45.0 58.0 57.9 1491-1531 bp 0.974359 86.6
rpl32-trnL_UAG_p4 TCGCACGCTGAACTTAATCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 1306-1406 bp 0.897436 85.0
rpl32-trnL_UAG_p5 TCGCACGCTGAACTTAATCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 1307-1407 bp 0.897436 85.0