Hotspot

trnN_GUU-ycf1

IRb · 109476-109809 · 334 bp

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Pi 0.0625
MarkerSeek score 69.30
Primer available yes
Label trnN_GUU-ycf1

Feature Metadata

label_name
trnN_GUU-ycf1
feature_type
igs
region
IRb
length_bp
334
variable_sites
104
pi
0.0625
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN_GUU-ycf1_p1 TAGCATAGCAAAGACGCGTT GGAAGAGATAAGAGGGCCCT 45.0 55.0 58.0 57.9 823-1649 bp 1.0 91.1
trnN_GUU-ycf1_p2 TAGCATAGCAAAGACGCGTT CCTTCTTCCATAACCCGAGC 45.0 55.0 58.0 58.0 851-1677 bp 0.974359 90.6
trnN_GUU-ycf1_p3 GGCAGAATAGCAGAGCAAGT CCTTCTTCCATAACCCGAGC 50.0 55.0 58.0 58.0 882-1708 bp 0.974359 90.3
trnN_GUU-ycf1_p4 GAGCGAGCCCTTTAATTCCA CCTTCTTCCATAACCCGAGC 50.0 55.0 58.0 58.0 587-1413 bp 0.974359 90.3
trnN_GUU-ycf1_p5 GTAACCCAGGCCAGAATCAG CCTTCTTCCATAACCCGAGC 55.0 55.0 58.0 58.0 999-1520 bp 0.923077 87.1