Hotspot

trnI_CAU-ycf2

IRb · 86982-87177 · 196 bp

Back to Paris

Pi 0.045
MarkerSeek score 63.60
Primer available yes
Label trnI_CAU-ycf2

Feature Metadata

label_name
trnI_CAU-ycf2
feature_type
igs
region
IRb
length_bp
196
variable_sites
59
pi
0.045
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI_CAU-ycf2_p1 GTTGGGCGCTTTAACCATTC AGGATTTTAATTGATGTCGTTTCA 50.0 29.2 58.0 55.4 203-628 bp 0.846154 40.6
trnI_CAU-ycf2_p2 AGTTGGGCGCTTTAACCATT AGGATTTTAATTGATGTCGTTTCA 45.0 29.2 58.4 55.4 204-629 bp 0.846154 40.6
trnI_CAU-ycf2_p3 TTGGGCGCTTTAACCATTCA AGGATTTTAATTGATGTCGTTTCA 45.0 29.2 58.4 55.4 202-627 bp 0.846154 40.6
trnI_CAU-ycf2_p4 GTTGGGCGCTTTAACCATTC GGATTTTAATTGATGTCGTTTCA 50.0 30.4 58.0 54.0 202-627 bp 0.846154 40.6
trnI_CAU-ycf2_p5 ATTGAACATTGGCGTGCATC AGGATTTTAATTGATGTCGTTTCA 45.0 29.2 57.7 55.4 259-684 bp 0.846154 40.2