Hotspot

rpoB-trnC_GCA

LSC · 27015-27880 · 866 bp

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Pi 0.0153
MarkerSeek score 60.50
Primer available yes
Label rpoB-trnC_GCA

Feature Metadata

label_name
rpoB-trnC_GCA
feature_type
igs
region
LSC
length_bp
866
variable_sites
64
pi
0.0153
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC_GCA_p1 CCAATTCTTCCGTCAAGCCT TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 57.5 987-1144 bp 1.0 83.5
rpoB-trnC_GCA_p2 TCCAATTCTTCCGTCAAGCC TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 57.5 988-1145 bp 1.0 83.4
rpoB-trnC_GCA_p3 CCAATTCTTCCGTCAAGCCT TTTGTTCATCAGGCGACACC 50.0 50.0 58.2 58.8 1024-1181 bp 1.0 81.4
rpoB-trnC_GCA_p4 TCCAATTCTTCCGTCAAGCC TTTGTTCATCAGGCGACACC 50.0 50.0 58.2 58.8 1025-1182 bp 1.0 81.3
rpoB-trnC_GCA_p5 CCAATTCTTCCGTCAAGCCT GACACCCGGATTTGAACTGG 50.0 55.0 58.2 58.8 1010-1167 bp 1.0 80.9