Hotspot

trnS_GCU-trnG_UCC

LSC · 8232-9379 · 1148 bp

Back to Paris

Pi 0.0094
MarkerSeek score 60.30
Primer available yes
Label trnS_GCU-trnG_UCC

Feature Metadata

label_name
trnS_GCU-trnG_UCC
feature_type
igs
region
LSC
length_bp
1148
variable_sites
77
pi
0.0094
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS_GCU-trnG_UCC_p1 CAATCCGACGCTTTAGTCCA AACCGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 787-1335 bp 1.0 76.1
trnS_GCU-trnG_UCC_p2 AATCCGACGCTTTAGTCCAC AACCGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 786-1334 bp 1.0 76.1
trnS_GCU-trnG_UCC_p3 GATAGAGCGGAGAGAGAGGG AACCGAAAGACCCCTTAACT 60.0 45.0 58.2 56.4 847-1395 bp 1.0 74.5
trnS_GCU-trnG_UCC_p4 CAATCCGACGCTTTAGTCCA AAACCGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 788-1336 bp 1.0 74.2
trnS_GCU-trnG_UCC_p5 AATCCGACGCTTTAGTCCAC AAACCGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 787-1335 bp 1.0 74.2