Hotspot

trnK-UUU-rps16

LSC · 4078-4834 · 757 bp

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Pi 0.0102
MarkerSeek score 69.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
757
variable_sites
44
pi
0.0102
unique_haplotype_count
32
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG GCGCTCAACCTACAGTAACT 50.0 50.0 58.6 57.6 845-952 bp 1.0 80.7
trnK-UUU-rps16_p2 TAGTCGCACTTAAAAGCCGA GCGCTCAACCTACAGTAACT 45.0 50.0 57.3 57.6 846-953 bp 1.0 79.4
trnK-UUU-rps16_p3 TCGCACTTAAAAGCCGAGTA GCGCTCAACCTACAGTAACT 45.0 50.0 57.3 57.6 843-950 bp 1.0 79.4
trnK-UUU-rps16_p4 CGCACTTAAAAGCCGAGTAC GCGCTCAACCTACAGTAACT 50.0 50.0 57.2 57.6 842-949 bp 1.0 79.0
trnK-UUU-rps16_p5 AGTCGCACTTAAAAGCCGAG GGCGCTCAACCTACAGTAAC 50.0 55.0 58.6 58.6 846-953 bp 1.0 78.5