primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCGCACTTAAAAGCCGAG	GCGCTCAACCTACAGTAACT	20	20	50.0	50.0	58.6	57.6	6.4	0.0	0.935945	4078	4834	845-952	1.000000	80.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TAGTCGCACTTAAAAGCCGA	GCGCTCAACCTACAGTAACT	20	20	45.0	50.0	57.3	57.6	3.0	0.0	1.094097	4078	4834	846-953	1.000000	79.4
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCGCACTTAAAAGCCGAGTA	GCGCTCAACCTACAGTAACT	20	20	45.0	50.0	57.3	57.6	3.0	0.0	1.094097	4078	4834	843-950	1.000000	79.4
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CGCACTTAAAAGCCGAGTAC	GCGCTCAACCTACAGTAACT	20	20	50.0	50.0	57.2	57.6	0.0	0.0	1.147606	4078	4834	842-949	1.000000	79.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGTCGCACTTAAAAGCCGAG	GGCGCTCAACCTACAGTAAC	20	20	50.0	55.0	58.6	58.6	6.4	7.6	1.213599	4078	4834	846-953	1.000000	78.5
trnS_GCU-trnG_UCC_p1	trnS_GCU-trnG_UCC	1	CAATCCGACGCTTTAGTCCA	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	58.0	56.4	0.0	0.0	1.640998	8232	9379	787-1335	1.000000	76.1
trnS_GCU-trnG_UCC_p2	trnS_GCU-trnG_UCC	2	AATCCGACGCTTTAGTCCAC	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	58.0	56.4	0.0	0.0	1.642386	8232	9379	786-1334	1.000000	76.1
trnS_GCU-trnG_UCC_p3	trnS_GCU-trnG_UCC	3	GATAGAGCGGAGAGAGAGGG	AACCGAAAGACCCCTTAACT	20	20	60.0	45.0	58.2	56.4	0.0	0.0	1.815199	8232	9379	847-1395	1.000000	74.5
trnS_GCU-trnG_UCC_p4	trnS_GCU-trnG_UCC	4	CAATCCGACGCTTTAGTCCA	AAACCGAAAGACCCCTTAACT	20	21	50.0	42.9	58.0	57.1	0.0	0.0	1.875121	8232	9379	788-1336	1.000000	74.2
trnS_GCU-trnG_UCC_p5	trnS_GCU-trnG_UCC	5	AATCCGACGCTTTAGTCCAC	AAACCGAAAGACCCCTTAACT	20	21	50.0	42.9	58.0	57.1	0.0	0.0	1.876509	8232	9379	787-1335	1.000000	74.2
rpoB-trnC_GCA_p1	rpoB-trnC_GCA	1	CCAATTCTTCCGTCAAGCCT	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	57.5	0.0	14.6	0.698720	27015	27880	987-1144	1.000000	83.5
rpoB-trnC_GCA_p2	rpoB-trnC_GCA	2	TCCAATTCTTCCGTCAAGCC	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	57.5	0.0	14.6	0.703889	27015	27880	988-1145	1.000000	83.4
rpoB-trnC_GCA_p3	rpoB-trnC_GCA	3	CCAATTCTTCCGTCAAGCCT	TTTGTTCATCAGGCGACACC	20	20	50.0	50.0	58.2	58.8	0.0	0.0	0.938794	27015	27880	1024-1181	1.000000	81.4
rpoB-trnC_GCA_p4	rpoB-trnC_GCA	4	TCCAATTCTTCCGTCAAGCC	TTTGTTCATCAGGCGACACC	20	20	50.0	50.0	58.2	58.8	0.0	0.0	0.943963	27015	27880	1025-1182	1.000000	81.3
rpoB-trnC_GCA_p5	rpoB-trnC_GCA	5	CCAATTCTTCCGTCAAGCCT	GACACCCGGATTTGAACTGG	20	20	50.0	55.0	58.2	58.8	0.0	0.7	1.008345	27015	27880	1010-1167	1.000000	80.9
trnI_CAU-ycf2_p1	trnI_CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	AGGATTTTAATTGATGTCGTTTCA	20	24	50.0	29.2	58.0	55.4	7.6	0.0	6.646853	86982	87177	203-628	0.846154	40.6
trnI_CAU-ycf2_p2	trnI_CAU-ycf2	2	AGTTGGGCGCTTTAACCATT	AGGATTTTAATTGATGTCGTTTCA	20	24	45.0	29.2	58.4	55.4	7.6	0.0	7.009789	86982	87177	204-629	0.846154	40.6
trnI_CAU-ycf2_p3	trnI_CAU-ycf2	3	TTGGGCGCTTTAACCATTCA	AGGATTTTAATTGATGTCGTTTCA	20	24	45.0	29.2	58.4	55.4	7.6	0.0	7.015660	86982	87177	202-627	0.846154	40.6
trnI_CAU-ycf2_p4	trnI_CAU-ycf2	4	GTTGGGCGCTTTAACCATTC	GGATTTTAATTGATGTCGTTTCA	20	23	50.0	30.4	58.0	54.0	7.6	0.0	7.044270	86982	87177	202-627	0.846154	40.6
trnI_CAU-ycf2_p5	trnI_CAU-ycf2	5	ATTGAACATTGGCGTGCATC	AGGATTTTAATTGATGTCGTTTCA	20	24	45.0	29.2	57.7	55.4	0.0	0.0	6.920353	86982	87177	259-684	0.846154	40.2
trnN_GUU-ycf1_p1	trnN_GUU-ycf1	1	TAGCATAGCAAAGACGCGTT	GGAAGAGATAAGAGGGCCCT	20	20	45.0	55.0	58.0	57.9	15.5	29.2	0.115336	109476	109809	823-1649	1.000000	91.1
trnN_GUU-ycf1_p2	trnN_GUU-ycf1	2	TAGCATAGCAAAGACGCGTT	CCTTCTTCCATAACCCGAGC	20	20	45.0	55.0	58.0	58.0	15.5	0.0	0.049656	109476	109809	851-1677	0.974359	90.6
trnN_GUU-ycf1_p3	trnN_GUU-ycf1	3	GGCAGAATAGCAGAGCAAGT	CCTTCTTCCATAACCCGAGC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.080603	109476	109809	882-1708	0.974359	90.3
trnN_GUU-ycf1_p4	trnN_GUU-ycf1	4	GAGCGAGCCCTTTAATTCCA	CCTTCTTCCATAACCCGAGC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.083460	109476	109809	587-1413	0.974359	90.3
trnN_GUU-ycf1_p5	trnN_GUU-ycf1	5	GTAACCCAGGCCAGAATCAG	CCTTCTTCCATAACCCGAGC	20	20	55.0	55.0	58.0	58.0	5.0	0.0	0.089196	109476	109809	999-1520	0.923077	87.1
ycf1_p1	ycf1	1	TAGCATAGCAAAGACGCGTT	CAAATGGAGTTGGGGTTGGA	20	20	45.0	50.0	58.0	58.0	15.5	0.0	0.012825	109810	111146	1319-2600	0.769231	82.7
ycf1_p2	ycf1	2	CCCGGAACTTCTTCGTAGTG	CAAATGGAGTTGGGGTTGGA	20	20	55.0	50.0	58.0	58.0	0.7	0.0	0.016073	109810	111146	853-2174	0.769231	82.6
ycf1_p3	ycf1	3	CCGGAACTTCTTCGTAGTGG	CAAATGGAGTTGGGGTTGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.016073	109810	111146	852-2173	0.769231	82.6
ycf1_p4	ycf1	4	GAGCGAGCCCTTTAATTCCA	CAAATGGAGTTGGGGTTGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.046629	109810	111146	1055-2336	0.769231	82.4
ycf1_p5	ycf1	5	GAGCTACTGAGGAACAACGG	CAAATGGAGTTGGGGTTGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.010573	109810	111146	939-2220	0.743590	81.7
ndhF-rpl32_p1	ndhF-rpl32	1	TGCATATTGATATGTATGTTCCA	AGAAAGAGCTTTTACTGCAGT	23	21	30.4	38.1	53.7	55.4	11.5	25.1	10.883754	113373	114147	849-974	1.000000	49.5
ndhF-rpl32_p2	ndhF-rpl32	2	TGCATATTGATATGTATGTTCCA	AAGAAAGAGCTTTTACTGCAGT	23	22	30.4	36.4	53.7	56.2	11.5	25.1	11.119561	113373	114147	850-975	1.000000	49.5
ndhF-rpl32_p3	ndhF-rpl32	3	TGCATATTGATATGTATGTTCCA	AGAAAGAGCTTTTACTGCAGTT	23	22	30.4	36.4	53.7	56.2	11.5	27.1	11.119561	113373	114147	849-974	1.000000	49.5
ndhF-rpl32_p4	ndhF-rpl32	4	ATGCATATTGATATGTATGTTCCA	AGAAAGAGCTTTTACTGCAGT	24	21	29.2	38.1	54.3	55.4	12.0	25.1	11.303980	113373	114147	850-975	1.000000	49.5
ndhF-rpl32_p5	ndhF-rpl32	5	TGCATATTGATATGTATGTTCCAT	AGAAAGAGCTTTTACTGCAGT	24	21	29.2	38.1	54.3	55.4	11.5	25.1	11.303980	113373	114147	849-974	1.000000	49.5
rpl32-trnL_UAG_p1	rpl32-trnL_UAG	1	ACTCCTTTTTGAATGGCGGT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.012454	114298	115132	1019-1053	0.974359	88.6
rpl32-trnL_UAG_p2	rpl32-trnL_UAG	2	ACTCCTTTTTGAATGGCGGT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.013825	114298	115132	1020-1054	0.974359	88.5
rpl32-trnL_UAG_p3	rpl32-trnL_UAG	3	ACTCCTTTTTGAATGGCGGT	TGGCGAAACCTTGGGTAAAA	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.073458	114298	115132	1491-1531	0.974359	86.6
rpl32-trnL_UAG_p4	rpl32-trnL_UAG	4	TCGCACGCTGAACTTAATCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.080947	114298	115132	1306-1406	0.897436	85.0
rpl32-trnL_UAG_p5	rpl32-trnL_UAG	5	TCGCACGCTGAACTTAATCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.082318	114298	115132	1307-1407	0.897436	85.0
ycf1_p1	ycf1	1	ATTTTGCGTCTTTGAGCACG	GAAAGCGGAAGAGGAAGAGG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.053694	128557	128676	291-432	0.923077	88.5
ycf1_p2	ycf1	2	CTGAATCCCGTCTGCTAACC	GAAAGCGGAAGAGGAAGAGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.075813	128557	128676	859-1135	0.923077	88.3
ycf1_p3	ycf1	3	CAAAGCCAGTTCTTCCTCGT	GAAAGCGGAAGAGGAAGAGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.132903	128557	128676	217-358	0.923077	87.9
ycf1_p4	ycf1	4	ATTTTGCGTCTTTGAGCACG	CCCAAGAAAAGCCAAACGTG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.184898	128557	128676	204-345	0.923077	87.4
ycf1_p5	ycf1	5	TCAAAGCCAGTTCTTCCTCG	GAAAGCGGAAGAGGAAGAGG	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.186970	128557	128676	218-359	0.923077	87.4
ycf1-trnN_GUU_p1	ycf1-trnN_GUU	1	GGAAGAGATAAGAGGGCCCT	TAGCATAGCAAAGACGCGTT	20	20	55.0	45.0	57.9	58.0	29.2	15.5	0.115336	130912	131245	823-1649	1.000000	91.1
ycf1-trnN_GUU_p2	ycf1-trnN_GUU	2	CCTTCTTCCATAACCCGAGC	TAGCATAGCAAAGACGCGTT	20	20	55.0	45.0	58.0	58.0	0.0	15.5	0.049656	130912	131245	851-1677	0.974359	90.6
ycf1-trnN_GUU_p3	ycf1-trnN_GUU	3	CCTTCTTCCATAACCCGAGC	GGCAGAATAGCAGAGCAAGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.080603	130912	131245	882-1708	0.974359	90.3
ycf1-trnN_GUU_p4	ycf1-trnN_GUU	4	CCTTCTTCCATAACCCGAGC	GAGCGAGCCCTTTAATTCCA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.083460	130912	131245	587-1413	0.974359	90.3
ycf1-trnN_GUU_p5	ycf1-trnN_GUU	5	CCTTCTTCCATAACCCGAGC	GTAACCCAGGCCAGAATCAG	20	20	55.0	55.0	58.0	58.0	0.0	5.0	0.089196	130912	131245	999-1520	0.923077	86.4
ycf2_p1	ycf2	1	TCAGCCAATTGTCTCGATCG	TCGAACCGAGAGATCCATGA	20	20	50.0	50.0	58.1	57.9	9.4	0.0	0.126221	151046	151165	743-806	1.000000	89.5
ycf2_p2	ycf2	2	TCAGCCAATTGTCTCGATCG	GGGGATACACGTTACACCAC	20	20	50.0	55.0	58.1	58.0	9.4	0.0	0.087774	151046	151165	1176-1392	1.000000	88.4
ycf2_p3	ycf2	3	AAGCCTCGGACCAATCAATC	GGGGATACACGTTACACCAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.058458	151046	151165	705-882	0.897436	81.7
ycf2_p4	ycf2	4	AAGCCTCGGACCAATCAATC	TCGAACCGAGAGATCCATGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.096905	151046	151165	278-296	0.897436	81.3
ycf2_p5	ycf2	5	AAGAAACGCAAACGAAGTGC	GGGGATACACGTTACACCAC	20	20	45.0	55.0	58.2	58.0	0.0	0.0	0.191754	151046	151165	748-925	0.897436	80.5
ycf2-trnI_CAU_p1	ycf2-trnI_CAU	1	AGGATTTTAATTGATGTCGTTTCA	GTTGGGCGCTTTAACCATTC	24	20	29.2	50.0	55.4	58.0	0.0	7.6	6.646853	153544	153739	203-628	0.846154	40.4
ycf2-trnI_CAU_p2	ycf2-trnI_CAU	2	AGGATTTTAATTGATGTCGTTTCA	TTGGGCGCTTTAACCATTCA	24	20	29.2	45.0	55.4	58.4	0.0	7.6	7.015660	153544	153739	202-627	0.846154	40.4
ycf2-trnI_CAU_p3	ycf2-trnI_CAU	3	GGATTTTAATTGATGTCGTTTCA	GTTGGGCGCTTTAACCATTC	23	20	30.4	50.0	54.0	58.0	0.0	7.6	7.044270	153544	153739	202-627	0.846154	40.4
ycf2-trnI_CAU_p4	ycf2-trnI_CAU	4	AGGATTTTAATTGATGTCGTTTCA	AGTTGGGCGCTTTAACCATT	24	20	29.2	45.0	55.4	58.4	0.0	7.6	7.009789	153544	153739	204-629	0.846154	40.3
ycf2-trnI_CAU_p5	ycf2-trnI_CAU	5	AGGATTTTAATTGATGTCGTTTCA	ATTGAACATTGGCGTGCATC	24	20	29.2	45.0	55.4	57.7	0.0	0.0	6.920353	153544	153739	259-684	0.846154	39.9