Hotspot

ndhE

SSC · 124700-125005 · 306 bp

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Pi 0

MarkerSeek score 21.60

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 21.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TGGAGCAGAGGCAATTTGTT	ATAGGTGCTATTGCTGTGGC	45.0	50.0	58.0	58.0	1079-1088 bp	1.0	80.7
ndhE_p2	TTGGAGCAGAGGCAATTTGT	ATAGGTGCTATTGCTGTGGC	45.0	50.0	58.0	58.0	1080-1089 bp	1.0	80.7
ndhE_p3	TTACACCCGTCCCAAGGTAT	ATAGGTGCTATTGCTGTGGC	50.0	50.0	58.0	58.0	1054-1063 bp	1.0	80.4
ndhE_p4	TGGAGCAGAGGCAATTTGTT	ACCGTTGGGAATGGGTTTAC	45.0	50.0	58.0	58.1	1301-1310 bp	1.0	80.1
ndhE_p5	TTGGAGCAGAGGCAATTTGT	ACCGTTGGGAATGGGTTTAC	45.0	50.0	58.0	58.1	1302-1311 bp	1.0	80.1